FIGURE 4.

CircLOC729852 exerts its oncogenic effects by suppressing miR‐769‐5p. (A) The target genes of miR‐769‐5p were predicted by targetscan and miRwalk 2.0 databases, and the intersection was obtained. (B) Expression levels of three candidate genes of miR‐769‐5p, IL‐10, CXCL15 and CCL1, after transfection with NC and inhibitor of BLCA. (C) Illustration of IL‐10 Wt and Mut luciferase reporter vectors. (D) The relative activity of luciferase in 293 T cells co‐transfected with IL‐10‐Mut or IL‐10‐Wt and miR‐769‐5p NC or inhibitor was measured by dual luciferase reporter method. (E) IL‐10 level in 20 paired BLCA and adjacent normal tissues. (F) IL‐10 level in BLCA cells transfected with NC, sh‐IL‐10 and Sh‐IL‐10‐2. (G) IL‐10 levels in BLCA cells after transfection with NC and rh‐IL‐10. (H) After transfection with NC, sh‐circ and sh‐circ+inhibitor, the EMT marker and IL‐10 levels in BLCA cells were detected by Western blotting. (I) After transfection with NC, sh‐circ and sh‐circ+rh‐IL‐10, rescue experiment was performed, and the BLCA cells' migration was examined by wound healing experiment. (J) After transfection with NC, sh‐circ and sh‐circ+rh‐IL‐10, rescue experiment was performed, and the BLCA cells' proliferation was examined by EdU assay. (K) After transfection with NC, sh‐circ and sh‐circ+rh‐IL‐10, rescue experiments were performed, and transwell migration assay was performed to examine the BLCA cells' migration.