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. 2024 Mar 20;28(7):e18225. doi: 10.1111/jcmm.18225

FIGURE 6.

FIGURE 6

CircLOC729852 promotes M2 macrophage polarization and recruitment via IL‐10. (A) The percentage of CD14 and CD68 positive cells was examined by RT‐qPCR and flow cytometry. (B) The percentage of CD86 and CD206 positive cells was examined by RT‐qPCR and flow cytometry. (C) M0 TAMs co‐cultured with BLCA cells transfected with NC, sh‐circ and sh‐circ+rh‐IL‐10 were performed rescue experiments, and western blotting was used to detect JAK2/STAT3 signalling pathway and IL‐10 expression level. (D) IL‐10 secretion levels in the supernatant of BLCA medium after transfection with NC and sh‐circ. (E) The percentage of CD86, TNF‐a, CD206 and CD163 positive cells was determined by RT‐qPCR and the percentage of CD86 and CD206 positive cells was examined by flow cytometry. The expression of CD86, TNF‐a, CD206 and CD163 in M0 TAMs cells induced by IL‐13 and IL‐4. (F) Rescue experiments were performed on M2‐Tams co‐cultured with BLCA cells transfected with NC, sh‐circ and sh‐circ +rh‐IL‐10, and the transwell assay was used to examine the migration of M2 TAMs. (G) IL‐10 secretion levels in the medium of M0 and M2 TAMs. (H) IL‐10 expression in BLCA cells co‐cultured with M0, M2 TAMs. (I) Migration ability of BLCA cells co‐cultured with M0, M2 TAMs.