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. 2023 Oct 11;3:e18. doi: 10.1017/S2633903X23000181

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© The Author(s) 2023

This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (http://creativecommons.org/licenses/by-nc-nd/4.0), which permits non-commercial re-use, distribution, and reproduction in any medium, provided that no alterations are made and the original article is properly cited. The written permission of Cambridge University Press must be obtained prior to any commercial use and/or adaptation of the article.

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Figure 3. — Schematic overview of the SPTBatch procedure for single-particle tracking along with subsequent motion trajectory analysis, cluster size, and single-step photobleaching analysis together with chemotaxis analysis in batch mode. (1) Extended trajectory analysis. Single-particle tracking analysis extending from TrackMate running in batch mode using multiple sets of files. (2) Motion Type Analysis. Trajectory analysis is executed to calculate short-time lag diffusion coefficient, diffusion coefficient, mean squared displacement curve, motion type classification, … (3) Cluster size analysis and single-step photobleaching analysis is run. (4) Chemotaxis and migration analysis to quantify chemotactic cell migration.