Table 1.
Description of cell line components used in the assays—parental cell line, cell line name, number of annotated images, size of pixels, fluorescent reporter protein (FRP), channel number, localization, and structural characterization.
| Parental cell line | Cell line | # Annotated samples | Pixel size (μM) | FRP | Channel # | Localization | Structural characterization |
|---|---|---|---|---|---|---|---|
| U2OS | CL1 | 50 | 1.24 | mCerulean-RAF | 0 | Constant (cytoplasm) | Cyto: Strong Nuclei: Weak |
| Venus-RAS | 1 | Constant (nuclei and cytoplasm) | Cyto: Weak Nuclei: Strong |
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| mCherry-ERK | 2 | Dynamic (nuclei and cytoplasm) | Cyto: Weak Nuclei: Weak |
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| CL2 | 28 | 0.43 | mCerulean-53BP1trunc | 0 | Constant (nuclei) | Cyto: Weak Nuclei: Strong |
|
| Venus-ATF6 | 1 | Dynamic (endoplasmic reticulum) | Cyto: Weak Nuclei: Strong |
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| H2B-mCherry | 2 | Constant (nuclei) | Cyto: Weak Nuclei: Strong |
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| MTS-miRFP | 3 | Dynamic (mitochondria) | Cyto: Weak Nuclei: Weak |
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| CL3 | 22 | 0.43 | H2B-TagBFP | 0 | Constant (nuclei) | Cyto: Weak Nuclei: Strong |
|
| Venus-LC3 | 1 | Dynamic (cytoplasm) | Cyto: Weak Nuclei: Weak |
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| MTS-mCherry | 2 | Dynamic (mitochondria) | Cyto: Weak Nuclei: Weak |
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| palm-miRFP | 3 | Dynamic (membrane) | Cyto: Weak Nuclei: Weak |
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| A375 | CL4 | 50 | 1.24 | mCerulean-RAF | 0 | Constant (cytoplasm) | Cyto: Strong Nuclei: Weak |
| Venus-RAS | 1 | Constant (nuclei and cytoplasm) | Cyto: Strong Nuclei: Weak |
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| mCherry-ERK | 2 | Dynamic (nuclei and cytoplasm) | Cyto: Strong Nuclei: Weak |
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| miRFP-MEK | 3 | Constant (cytoplasm) | Cyto: Strong Nuclei: Weak |
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| CL5 | 50 | 1.24 | AKT-KTR-mCerulean | 0 | Dynamic (nuclei and cytoplasm) | Cyto: Weak Nuclei: Weak |
|
| ERK-KTR-Venus | 1 | Dynamic (nuclei and cytoplasm) | Cyto: Strong Nuclei: Weak |
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| miRFP-LC3 | 2 | Dynamic (cytoplasm) | Cyto: Strong Nuclei: Weak |
Note. For RAS and RAF proteins, different isoforms and/or mutations were tagged.
Abbreviations: KTR, kinase translocation reporter; MTS, mitochondria targeting signal; palm, palmitoylation signal; 53BP1trunc, 53BP1trunc(1220-1711).