| Antibodies |
|
| Rabbit polyclonal anti-NPY |
BMA Biomedicals |
(Cat# T-4070, RRID:AB_518504) |
| Chicken polyclonal anti-GFP |
Abcam |
(Cat# ab13970, RRID:AB_300798) |
| Rabbit polyclonal anti-tyrosine hydroxylase |
Millipore |
(Cat# ab152, RRID:AB_390204) |
| Mouse monoclonal anti-tyrosine hydroxylase |
Sigma-Aldrich |
(Cat# T2928, RRID:AB_477569) |
| Sheep polyclonal anti-tyrosine hydroxylase |
Millipore |
(Cat# ab1542, RRID:AB_90755) |
| Rabbit monoclonal anti-RFP |
Abcam |
(Cat# ab185921, RRID:AB_2934052) |
| Rabbit polyclonal anti-cFos |
Abcam |
(Cat# ab190289; RRID:AB_2737414) |
| Rabbit monoclonal anti-cFos |
Abcam |
(Cat# ab222699, RRID:AB_2891049) |
| Donkey anti-Rabbit IgG Alexa Flour 647 |
Thermo Fisher Scientific |
(Cat# A-31573, RRID:AB_2536183) |
| Donkey anti-Chicken IgG Alexa Flour 488 |
Sigma-Aldrich |
(Cat# SAB4600031, RRID:AB_2721061) |
| Donkey anti-Sheep IgG Alexa Flour 546 |
Thermo Fisher Scientific |
(Cat# A-21098, RRID:AB_2535752) |
| Donkey anti-Sheep IgG Alexa Flour 647 |
Abcam |
(Cat# ab150179, RRID:AB_2884038) |
| Goat anti-Mouse IgG1 Alexa 647 |
Thermo Fisher Scientific |
(Cat# A-21240, RRID:AB_2535809) |
| Goat anti-Rabbit IgG Alexa 647 |
Thermo Fisher Scientific |
(Cat# A-21244, RRID:AB_2535812) |
| Goat anti-Rabbit IgG Alexa 546 |
Thermo Fisher Scientific |
(Cat# A-11035, RRID:AB_2534093) |
|
| Chemicals, peptides, and recombinant proteins |
|
| Glycogen, RNA grade |
Thermo Fisher Scientific |
Cat# R0551 |
| Trizol |
Thermo Fisher Scientific |
Cat# 15596018 |
| Maxima SYBR Green/ROX qPCR Master Mix (2X) |
Thermo Fisher Scientific |
Cat# K0222 |
| Paraformaldehyde, reagent grade |
Sigma-Aldrich |
Cat# P6148-500G |
| Bouin’s solution |
Sigma-Aldrich |
Cat# HT10132- 1L |
| DAPI |
Roche |
Cat# 10236276001 |
| ProLong Gold Antifade Mountant |
Thermo Fisher Scientific |
Cat# P36930
|
| Fluoromount Mounting Media |
Sigma-Aldrich |
Cat# F4680- 25mL |
| Permount Mounting Media |
Fisher Scientific |
Cat# SP11500 |
| Hydrogen peroxide solution, 30%in H2O, ACS reagent |
Sigma-Aldrich |
Cat# 216763 |
| Heparin sodium salt |
Sigma-Aldrich |
Cat# H3393 |
| Isoflurane |
MWI |
Cat# 502017 |
| Insulin |
Novolin-R |
Cat# A10005804 |
| D-(+)-Glucose, ACS reagent |
Sigma-Aldrich |
Cat# G5767-25G |
| Histopaque(R)-1077 |
Sigma-Aldrich |
Cat#10771 |
| Histopaque(R)-1119 |
Sigma-Aldrich |
Cat#11191 |
| Collagenase P |
Sigma-Aldrich |
Cat#11249002001 |
| Aprotinin |
Sigma-Aldrich |
Cat#A1153 |
|
| Critical commercial assays |
|
| Superscript IV First Strand Synthesis System |
Thermo Fisher Scientific |
Cat# 18091050 |
| Ultra-Sensitive Mouse Insulin ELISA Kit |
Crystal Chem |
Cat# 90080 |
| PicoGreen Kit |
Thermo Fisher Scientific |
Cat#P7581 |
| Norepinephrine High Sensitive ELISA Kit |
Rocky Mountain Diagnostics |
Cat# BA E–5200R |
| Mercodia Glucagon 10ul ELISA |
Mercodia |
Cat#10-1281-01 |
|
| Experimental models: Organisms/strains |
|
| Mouse: B6.Cg-Npytm1(cre)Zman/J
|
The Jackson Laboratory |
RRID:IMSR_JAX:027851 |
| Mouse: B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
|
The Jackson Laboratory |
RRID:IMSR_JAX:006148 |
| Mouse: DBH-Cre
|
Quach et al.36
|
N/A |
| Mouse: B6.Cg-Npytm1.1(flpo)Hze/J
|
The Jackson Laboratory |
RRID:IMSR_JAX:030211 |
| Mouse: B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J
|
The Jackson Laboratory |
RRID:IMSR_JAX:021875 |
| Mouse: NPYfl/fl
|
Wee et al.42
|
N/A |
| Mouse: C57Bl/6J (Strain background) |
The Jackson Laboratory |
RRID:IMSR_JAX:000664 |
|
| Oligonucleotides |
|
|
Npy_F:5′-AGAGATCCAGCCCTGAGACA-3′ |
This paper |
qPCR primers |
|
Npy_R:5′-GATGAGGGTGGAAACTTGGA-3′ |
This paper |
qPCR primers |
|
Th_F:5′-AATCCACCACTTAGAGACCCG |
This paper |
qPCR primers |
|
Th_R:3′-CTTGGTGACCAGGTGGTGAC |
This paper |
qPCR primers |
|
18s_F:5′-CGCCGCTAGAGGTGAAATTC |
This paper |
qPCR primers |
|
18s_R:5′-TTGGCAAATGCTTTCGCTC |
This paper |
qPCR primers |
|
Npyfl/fl_F:5′-GCCTCCGTGCCTCTTATCTT-3′ |
Wee et al.42
|
Genotyping primers |
|
Npyfl/fl_R:5′-GATTTTGGAGGCAACAGAGC-3′ |
Wee et al.42
|
Genotyping primers |
|
B6.Cg-Npytm1(cre)Zman/J_F: 5′-TCC ATG ATT TGC CTC TTG TG -3′ |
The Jackson Laboratory |
Genotyping primers |
|
B6.Cg-Npytm1(cre)Zman/J_R: 5′-ACA CCG GCC TTA TTC CAA G -3′ |
The Jackson Laboratory |
Genotyping primers |
|
B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J_F: 5′-AGG GCG AGG AGC TGT TCA-3′ |
The Jackson Laboratory |
Genotyping primers |
|
B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J_R: 5′-TGA AGT CGA TGC CCT TCA G -3′ |
The Jackson Laboratory |
Genotyping primers |
|
DBH-Cre_F:5′-CTG CCA GGG ACA TGG CCA GG-3′ |
Quach et al.36
|
Genotyping primers |
|
DBH-Cre_R:5′- GCA CAG TCG AGG CTG ATC AGC -3′ |
Quach et al.36
|
Genotyping primers |
|
B6.Cg-Npytm1.1(flpo)Hze/J_F: 5′-TGC TGC TGT TCT CCG TAG C-3′ |
The Jackson Laboratory |
Genotyping primers |
|
B6.Cg-Npytm1.1(flpo)Hze/J_R: 5′-ACA CCG GCC TTA TTC CAA G-3′ |
The Jackson Laboratory |
Genotyping primers |
|
B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J_F:5′-CTG TTC CTG TAC GGC ATG G-3′ |
The Jackson Laboratory |
Genotyping primers |
|
B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J_R:5′-GGC ATT AAA GCA GCG TAT CC -3′ |
The Jackson Laboratory |
Genotyping primers |
|
| Software and algorithms |
|
| Ponemah Software |
N/A |
https://www.datasci.com/products/software/ponemah
|
| Anilogger Manager software |
N/A |
https://www.animals-monitoring.fr/wp-content/uploads/2020/06/P120GUI004-Quick.Start_.Anilogger.system.EN_.pdf
|
| Imaris |
Oxford |
https://imaris.oxinst.com
|
| ImageJ |
N/A |
https://ImageJ.nih.gov/ij/
|
|
ZEN 2012 (blue edition)
|
N/A |
https://www.zeiss.com/microscopy/int/home.html
|
| GraphPad Prism9 |
N/A |
https://www.graphstats.net/graphpad-prism
|
| BioRender |
N/A |
www.biorender.com
|
