Figure 1.
Early retinal organoid development (day 10–35) reveals key features of human retinogenesis, starting with optic vesicle formation, followed by the emergence of RPCs and RGCs
(A‒C) UMAP plots of scRNA-seq in ROs at day 10 (A), day 20 (B), and day 35 of differentiation (C).
(D‒I) Spatial localization of the individual (D‒F) and pooled (G‒I) clusters in ROs at day 10 (D and G), day 20 (E and H) and day 35 (F and I) of differentiation acquired from ST analyses. Cluster annotations and highly expressed markers are shown in Table S1 for scRNA-seq data and in Table S2 for ST data. For scRNA-seq 144 ROs were used at day 10/20 and 96 ROs at day 35. 40 ROs were used at day 10, 30 ROs at day 20 and 25 ROs at day 35 for ST. The scRNA-seq and ST were performed once at each time point.