Figure 2.
Mid organoid development (day 45–90) reveals the presence of pCM and specific localization of RPCs, NRPCS, RGCs and photoreceptor precursors
(A‒C) scRNA-Seq UMAPs of hiPSC-derived ROs at day 45 (A), day 60 (B) and day 90 (C).
(D‒I) Individual (D‒F) and pooled (G‒I) cluster location in ROs at day 45 (D and G), day 60 (E and H) and day 90 (F and I) of differentiation identified from the ST analyses. Cluster annotations and highly expressed markers are shown in Table S1 for scRNA-seq data and in Table S2 for ST data. For scRNA-seq 96 ROs were used for all days. 20 ROs were used at day 45/60 and 10 ROs at day 90 for ST. The scRNA-seq and ST were performed once at each time point.