Figure 4.

RPC identification and their developmental trajectories during RO development

(A) UMAP plot of integrated scRNA-seq of ROs during development from day 10 to day 210 of RO differentiation. Cluster annotations and highly expressed marker genes are shown in Table S4. For ease of visualization, clusters of the same cell type are shown by the same color.

(B) Dot plot presentation of highly expressed key marker for each retinal cell type in the integrated scRNA-seq data.

(C) Individual selected clusters of integrated scRNA-seq retinal cells (A) used for pseudotime analyses. Highly expressed markers for each cluster along the pseudotime trajectory are shown in Table S4.

(D and E) Pseudotime analysis demonstrating transition from pCM to early and late RPCs followed by T1 which further commits to either T2 or T3 transient neurogenic progenitors.

(F) Gene expression heatmap, indicating similarities in gene expression patterns between the pCM and early RPCs, and between T1 and T3 transient neurogenic progenitors.

Related to Table S4.