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Activation of virus-specific memory CTL response by the polyepitope protein targeted to the lysosomal compartment. Polyclonal effectors from an HLA DR1-positive donor were generated by restimulating peripheral blood lymphocytes with Vacc.ER-POLY-LAMP. Target cells were autologous LCLs either infected with recombinant vaccinia virus constructs or sensitized with the indicated peptide (10 μmol/ml). Effector/target ratios of 15:1 and 30:1 were used in the assay.
