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. 2023 Aug 11;95(8):e29025. doi: 10.1002/jmv.29025

Figure 2.

Figure 2

Depletion of CREB1 inhibits the proliferation, migration and EMT of cervical cancer cells in vitro. (A) Western blot analysis of CREB1 in indicated cell lines transfected with a pool of four siRNAs targeting CREB1 (siCREB1). GAPDH loading control; (B) qPCR analysis of CREB1 in indicated cell lines transfected with siCREB1; (C, D) Cell proliferation analysed by cell growth curve in indicated cell lines transfected with siCREB1, or A‐CREB, a dominant negative inhibitor for CREB1; (E,F) Representative images for colony formation assay performed in indicated cell lines transfected with siCREB1 or A‐CREB. Quantification of (E) as shown in (F); (G) Quantification of wound closure as an evaluation for cell migration in indicated cell lines transfected with siCREB1 or A‐CREB; (H) qPCR analysis of EMT markers in indicated cell lines transfected with siCREB1; (I) Western blot analysis of EMT markers in indicated cell lines transfected with siCREB1. Data shown are mean ± SD, n ≥ 3. *p < 0.05; **p < 0.01; ***p < 0.001. EMT, epithelial to mesenchymal transition; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; siRNA, small interfering RNA.