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. 2023 Aug 11;95(8):e29025. doi: 10.1002/jmv.29025

Figure 5.

Figure 5

miR‐203a overexpression attenuates cell proliferation by directly targeting CREB1. (A) qPCR analysis of miR‐203a in cervical cytology samples from patients with different CIN grades; (B) qPCR analysis of miR‐203a expression in CC cell lines compared to NHK; (C) qPCR analysis of miR‐203a in HPV18‐transformed NHK; (D, E) Western blot and qPCR analysis in indicated cells transfected with miR‐203a mimic; (F) Schematic of predicted miR‐203a binding sites within CREB1 3′‐UTR and point mutation introduced, fused to luciferase reporter system; (G) Dual luciferase reporter analysis of CREB1 3′‐UTR (WT and Mut) ‐controlled activity in HEK293T with overexpressing miR‐203a mimic; (H, I) Western blot and qPCR analysis of CREB1 in indicated cells co‐transfected with miR‐203a mimic and CREB1; (J–L) Cell proliferation and clonogenicity assessed by cell growth curve and colony formation assay in indicated cells co‐transfected with miR‐203a mimics and CREB1. Data shown are mean ± SD, n = 3. *p < 0.05; **p < 0.01; ***p < 0.001. CC, cervical cancer; HPV, human papillomaviruses; UTR, untranslated region.