FIG. 2.

Immunoblot of HCMV rp130 antigen for detection of the HCMV rp130 synthesized in insect cells infected with recombinant baculovirus-UL56. Infected cells were fractionated, and aliquots of each fraction were separated by SDS-PAGE followed by immunoblotting with a specific antiserum against p130. Lanes: 1, supernatant fraction from recombinant baculovirus-UL56-infected H5 cells; 2, sediment from recombinant baculovirus-infected H5 cells; 3, supernatant from wild-type baculovirus-infected H5 cells; 4, sediment from wild-type baculovirus-UL56-infected H5 cells. Molecular mass standards (M) are indicated on the left; the position of rp130 proteins is indicated by an arrow.