FIG. 5.

Effects of the incubation time and protein concentration on the enzyme activity. (A) Nuclease reaction mixtures were incubated at 37°C for the indicated time, and reactions were stopped by addition of proteinase K. Lanes: 1, pON205 alone; 2, pON205 treated with HindIII; 3, pON205 plus incubation of rp130 for 0 s; 4, incubation with rp130 for 30 s; 5, incubation for 15 min; 6, incubation for 40 min. (B) Nuclease reactions were performed for 40 min at 37°C prior to proteinase K treatment. Lanes: 1, pON205 alone; 2, pON205 treated with HindIII; 3, pON205 plus incubation with 5 μg of rp130; 4, incubation with 0.5 μg of rp130; 5, incubation with 0.25 μg of rp130; 6, pON205 plus incubation with 5 μg of extract of wild-type baculovirus; 7, incubation with 0.5 μg of wild-type extract; 8, incubation with 0.25 μg of wild-type extract; 9, pON205 plus 5 μg of mock-infected extract; 10, incubation with 0.5 μg of mock-infected extract; 11, incubation with 0.25 μg of mock-infected extract. Positions of open circular DNA molecules (a), linear molecules (b), and supercoiled molecules (c) are indicated.