Fig. 4.

Size distribution and morphology of purified starch granules from developing grains of Ttparc6‐2 durum wheat mutants. (a–d) Size distribution of purified starch granules from developing (12, 16, 21 day after flowering – DAF) and mature grain. The volume of granules at each diameter relative to the total granule volume was quantified using a Coulter counter. Values represent mean (solid line) ± SEM (shading) of three biological replicates using grains harvested from separate plants. (e–l) Scanning electron microscopy of purified starch granules. Bar, 10 μm. (m–t) Polarised light images of purified starch granules. Bar, 10 μm. WT, wild‐type.