Fig 2.

CzcS/CzcR induces the expression of ptrA and PA2807 during Zn excess. (A and B) Relative expression of the ptrA and PA2807 genes in the ΔczcR and ΔczcR (czcR) strains compared to the WT strain when they were cultured in the absence (A) or the presence (B) of Zn. (C) Electrophoretic mobility shift assays (EMSAs) showed the binding ability of CzcR at the promoters of ptrA and PA2807. (D and E) Expression of PptrA-lux (D) and PPA2807-lux (E) was measured in the WT and ΔczcR strains when they were cultured in the absence or presence of Zn. Statistical significance was calculated compared to the WT group based on one-way analysis of variance (ANOVA) (A and B) or two-way ANOVA (D and E) (n.s., not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001).