FIGURE 8.

CD81 is a microglial‐enriched protein expressed in neuroinflammation. (a) tSNE plot showing West Nile virus‐infected brains with resident and infiltrating CD45⁺ leukocyte populations overlaid. (b) Violin plots showing the expression of CD81 on cell populations identified on the tSNE plot. (c) Histogram showing the expression of CD81 by microglia relative to infiltrating macrophage populations and fluorescence minus one (FMO) for CD81 in infected brains. See also Figures S3 and S4 showing CD81 expression by leukocyte populations in the bone marrow and spleen, respectively. (d) Heatmap showing the expression of markers on cell populations shown in (a). (e) FACs plot showing the expression of CD81 and CD45 on microglia and other CD45⁺ leukocytes in infected brains. (f) Histogram showing the expression of CD81 on microglia from mock and infected brains at day 7 post infection