. 2023 Dec 9;102(2):117–130. doi: 10.1111/imcb.12714

Table 1.

Proportion inhibition by PD‐1 ligand fusion proteins at the peak of the response.

	α‐CD3 + PD‐L1.Fc	α‐CD3 + PD‐L2.Fc	Versus α‐CD3 only	Versus α‐CD3 + Ctrl.Fc	With α‐IL‐2	With α‐CD28	Genotype	Peak TP (h)	Total cell number (mean)		Proportion inhibition	Difference	P
	α‐CD3 + PD‐L1.Fc	α‐CD3 + PD‐L2.Fc	Versus α‐CD3 only	Versus α‐CD3 + Ctrl.Fc	With α‐IL‐2	With α‐CD28	Genotype	Peak TP (h)	Control	PD‐L.Fc	Mean ± standard error of the mean	Difference	P
Figure 1	●	○	●	○	●	●	WT	65.6	7164	4605	0.36 ± 0.08	0.06	0.525
Figure 1	●	○	●	○	●	●	PD‐1^−/−	65.6	15 632	10 869	0.30 ± 0.04	0.06	0.525
Figure 3	●	○	○	●	●	●	WT	48.7	10 281	9198	0.11 ± 0.06	−0.13	0.180
Figure 3	●	○	○	●	●	●	PD‐1^−/−	64.0	15 460	11 683	0.24 ± 0.05	−0.13	0.180
Figure 4a	●	○	○	●	○	●		n/a
Figure 4a	●	○	○	●	○	●		n/a
Figure 4b	●	○	○	●	●	○	WT	47.0	2137	1661	0.22 ± 0.03	0.03	0.544
Figure 4b	●	○	○	●	●	○	PD‐1^−/−	47.0	3264	2627	0.19 ± 0.03	0.03	0.544
Figure 5	○	●	○	●	●	●	WT	71.2	6239	3600	0.42 ± 0.08	0.15	0.154
Figure 5	○	●	○	●	●	●	PD‐1^−/−	71.2	20 235	14 794	0.27 ± 0.04	0.15	0.154

Unpaired t‐tests were performed on the proportion inhibition by Fc fusion protein in WT compared with PD‐1^−/− T‐cell responses.

IL, interleukin; PD‐1, programmed death receptor 1; PD‐L1, PD‐1 ligand 1; PD‐L1.Fc, PD‐L1 coupled to the Fc portion of IgG1; TP, time point; WT, wild type.