Table 1.
Heatmap of endpoint conversion for transamination of aldol panel 3–5 with (R)‐selective in‐house TAs and previously identified minimal panel of the commercial TA library. Amine product: light green: conv. <20%, green: conv. 20–60% dark green: conv. >60%. Conditions: lyophilized whole cells in case of ArthrTA or AtTA (4 mg) or CFE (5 mg), 3–5 (25 mM), d‐ or l‐alanine (375 mM), PLP (1 mM), Kpi buffer (50 mM, pH 7), total volume 200 μL, rt, horizontal shaking, 24 h.
|
|
Amino donor |
3 a |
3 b |
3 c |
4 a |
4 b |
4 c |
5 a |
5 b |
5 c |
|---|---|---|---|---|---|---|---|---|---|---|
|
ArthrTA |
d‐alanine |
|
|
|
|
|
|
|
|
|
|
AtTA |
d ‐alanine |
|
|
|
|
|
|
|
|
|
|
TA26 |
l‐alanine |
42,739 |
|
|
|
|
|
|
|
|
|
TA31 |
l‐alanine |
39,633 |
|
|
|
|
|
|
|
|
|
TA33 |
l‐alanine |
5,033 |
|
|
|
|
|
|
|
|
|
TA81 |
l‐alanine |
41,361 |
|
|
|
|
|
|
|
|
|
TA93 |
l‐alanine |
49,994 |
|
|
|
|
|
|
|
|
|
TA166 |
l‐alanine |
38,621 |
|
|
|
|
|
|
|
|
|
TA169 |
l‐alanine |
37,283 |
|
|
|
|
|
|
|
|
|
TA187 |
l‐alanine |
4,061 |
|
|
|
|
|
|
|
|
|
TA188 |
l‐alanine |
15,66 |
|
|
|
|
|
|
|
|
|
TA189 |
l‐alanine |
65,981 |
|
|
|
|
|
|
|
|
|
TA191 |
l‐alanine |
53,75 |
|
|
|
|
|
|
|
|
|
TA192 |
l‐alanine |
18,849 |
|
|
|
|
|
|
|
|