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. 2024 Mar 19;12(3):e008402. doi: 10.1136/jitc-2023-008402

Figure 2.

Figure 2

Shortlisted circRNA expression in CRC cell lines and clinical samples. (A) Expression of shortlisted circRNA in CRC (n=28) and normal (n=25) samples in MiOncoCirc data set. (B) Expression of shortlisted circRNA in CRC cell lines by RT-qPCR using divergent primers (n=4 in HCT116 and LS513, n=3 in SW480 and HT29). (C) Sanger sequencing of qPCR products showing the back splicing junction of circRNA. (D) Relative expression of shortlisted circRNA in six pairs of tumor and normal samples from patients with CRC by RT-qPCR. Empirical Bayes moderated t-statistics from edgeR was used in (A). The paired two-sided Student’s t-test was used in (D). The boxes in boxplots represented the median ±1 quartile, with the whiskers extending to the largest or smallest non-outlier value within 1.5 times the IQR from the third quartile and first quartile, respectively. BSJ, back-splicing junction; circRNA, circular RNA; CRC, colorectal cancer; RT-qPCR, quantitative real-time PCR; ACTB, actin beta.