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. 2023 Mar 16;601(22):5093–5106. doi: 10.1113/JP284047

Figure 3. HIIT exercise induces an increase in the number of circulating tetraspanin positive small extracellular vesicles and alters tetraspanin expression.

Figure 3

A, the number of plasma tetraspanin positive sEVs in pre‐ and post‐exercise, platelet‐free plasma represented by the summed fluorescence counts of CD9, CD63 or CD81 fluorescent antibodies across all capture spots. B, SP‐IRIS assessment of CD41a+ sEV number in pre‐ and post‐exercise. C, tetraspanin protein expression in pre‐ vs. post‐exercise plasma represented by summed mean fluorescence intensity of CD9, CD63 and CD81 antibodies across all capture spots. D, representative images of CD9 (CF488), CD63 (CF647) and CD81 (CF555) fluorescence signalling for each capture spot. n = 7. P‐values for fold change were determined with Student's paired t test. [Colour figure can be viewed at wileyonlinelibrary.com]