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. 2023 Dec 16;168(2):100–114. doi: 10.1111/jnc.16029

FIGURE 1.

FIGURE 1

AQP4‐eGFP translocation in HEK293 cells following dynasore and filipin treatment. HEK293 cells were transfected with AQP4‐eGFP and incubated in the presence of filipin and dynasore as described in the methods. AQP4‐eGFP localisation was imaged by live fluorescence microscopy in an isotonic solution at 30 s, at 5 min following exposure to a hypotonic medium, and at 5 min following return back to isotonic medium. (a) representative schematic of how HEK293 transfected with AQP4‐eGFP were analysed to determine relative membrane expression (RME). (b) RME quantification of AQP4‐eGFP in control and dynasore‐treated cells. (c) RME quantification of AQP4‐eGFP in control and filipin‐treated cells. Mean RME for AQP4‐eGFP averaged over three profiles/cell of at least 10 cells per condition in each repeat in three experimental repeats, n = 3. For each repeat, p values are from one‐way analysis of variance followed by Bonferroni's correction. All data are presented as mean ± SEM.