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Fragments of different sizes amplified to determine integrity of ZEG‐extracted gDNA. DNA was ZEG extracted at NF 25, NF 37, NF 42, and NF 45, amplified with primer pair A (150 bp), B (260 bp), C (550 bp) & D (800 bp) and resolved on a 1% agarose gel. Contrast was adjusted to reveal the low intensity spurious bands
