FIGURE 6.
Long term cigarette smoke (CS)‐induced systemic neutrophilia and vascular dysfunction were attenuated by apocynin treatment. Mice were exposed to CS or room air (sham) for 8 weeks with i.p. injection of apocynin (5 mg·kg−1·day−1) or vehicle (saline). Change in body weight (a), alterations in body lean (b) and fat (c) mass. Haematology analysis of total white blood cell (d), monocytes (e), neutrophils (f), lymphocytes (g) and haematocrit (h). Cumulative concentration response curves and EC50 analysis to acetylcholine and sodium nitroprusside (i; 1 × 10−8M to 1 × 10−5M) to examine endothelial‐dependent and endothelial‐independent vasodilation in mouse thoracic aorta, respectively. Data are expressed as mean + SEM (n = 8 mice per group) and analysed by two‐way ANOVA with multiple comparisons and Tukey post hoc test. *P < 0.05, denotes differences between the compared groups.