FIGURE 2.

Intact T‐cell responses to vaccination, irrespective of seroconversion status. (A) Interferon gamma (IFNγ) responses to spike protein‐derived peptide pools, normalised to spot‐forming units (SFU) per 10⁶ peripheral blood mononuclear cells (PBMCs). Negative values were given a value of 1 SFU for the purposes of the logarithmic scale. ELISpot assays were run for all samples where viable PBMCs were available (n = 41, 53, 36, 30, 31, at each timepoint respectively). (B) IFNγ responses to the omicron mutation peptide pool pre‐ and post‐third dose, and post‐fourth dose (n = 35, 30, 31, respectively). (C–G) IFNγ responses to WT spike protein peptides for each timepoint, divided into seropositive and seronegative patients. ELISpot results without matched serological data were excluded from this analysis. Dashed lines indicate the threshold for a positive response, calculated as three standard deviations above the mean of all unstimulated wells. Significance is shown on each graph as calculated using the Mann–Whitney U test, with values of p = 0.0133 and p = 0.0297 for the Pre‐ and Post‐2nd dose timepoints respectively.