TABLE 2.
Reagents used for OMIP
| Specificity | Fluorochrome | Clone | Purpose |
|---|---|---|---|
| CD45 | BUV395 | 30‐F11 | Pan leukocytes |
| IL‐7Rα | PE‐Cy5 | SB/199 | Pan ILC |
| PLZF | PE | MAGS21F7 | ILCP |
| IL‐18Rα | PerCP‐eFluor 710 | P3TUNYA | ILCP and ILC1 |
| T‐bet | BV421 | 4B10 | ILC1 and ILC3 subsets |
| NKp46 | PE/Dazzle 594 | 29A1.4 | ILC1, ILC3 subsets and NK cells |
| CD49a | BUV496 | Ha31/8 | ILC1 subsets and trNK cells |
| TRAIL | PE‐Cy7 | N2B2 | ILC1 subsets |
| IFN‐γ | BUV737 | XMG1.2 | ILC1 and NK cells |
| GATA‐3 | BB700 | L50‐823 | ILC2 |
| KLRG1 | BV480 | 2F1 | ILC2 |
| ST2 | R718 | U29‐93 | ILC2 |
| IL‐5 | APC | TRFK5 | ILC2 |
| IL‐13 | eFluor 450 | eBio13A | ILC2 |
| RORγt | BV786 | Q31‐378 | ILC3 |
| CCR6 | BV711 | 140706 | ILC3 subsets |
| IL‐17A | BV650 | TC11‐18H10.1 | ILC3 |
| IL‐22 | Alexa Fluor 647 | Poly5164 | ILC3 |
| CD49b | eFluor 506 | DX5 | NK cells |
| Ki‐67 | Alexa Fluor 532 | SolA15 | Proliferating cells |
| B220 | FITC | RA3‐6B2 | B cell subsets (Lin dump) |
| CD3 | FITC | 145‐2C11 | T cell subsets (Lin dump) |
| CD4 | FITC | GK1.5 | T cell subsets (Lin dump) |
| CD5 | FITC | 53‐7.3 | T cell and B cell subsets (Lin dump) |
| CD11b | FITC | M1/70 | Myeloid/NK/Granulocytes (Lin dump) |
| CD11c | FITC | N418 | Myeloid cells/Granulocytes (Lin dump) |
| CD19 | FITC | 1D3/CD19 | B cell subsets (Lin dump) |
| F4/80 | FITC | BM8 | Macrophages (Lin dump) |
| FcεR1α | FITC | MAR‐1 | Mast cells and Basophils (Lin dump) |
| Gr‐1 | FITC | RB6‐85C | Myeloid cells/Granulocytes (Lin dump) |
| TCRβ | FITC | H57‐597 | αβ T‐cells (Lin dump) |
| TCRγδ | FITC | UC7‐13D5 | γδ T‐cells (Lin dump) |
| Ter119 | FITC | TER‐119 | Red blood cells (Lin dump) |
| Dead cells | LIVE/DEAD™ Blue | ‐ | Viable cells |