FIGURE 3.

Direct targeting of SLC25A5 with the selective inhibitor PENAO significantly reduces NB cell viability. (A) Cell viability curves of NB and normal lung fibroblast cell lines upon PENAO treatment (0‐20 μM) for 72 h, cell lines are ranked according to IC₅₀. (B) Average IC₅₀'s of cell lines subcategorised by mutations in the TP53 pathway (TP53, CDKN2A). (C) PENAO cytotoxicity (IC₅₀) in neuroblastoma cell lines stably transduced with an shRNA construct targeting TP53 mRNA. (D) PENAO cytotoxicity (IC₅₀) in SH‐EP clones stably expressing TP53 cDNA constructs with point mutations in the DNA‐binding domain (p.C135R & p.C135P mutants) or empty vector controls (EV1/EV2). Error bars represent the SE of the mean of at least three independent biological replicates