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. 2023 Jul 3;120(10):3079–3091. doi: 10.1002/bit.28470

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© 2023 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(a) Immunocytochemistry of iPSC‐derived astrocytes in 2D culture and stained for the astrocyte markers S100β (green), GFAP (red), and nuclei counterstained with DAPI (blue). (b) Viability of iPSC‐derived astrocytes 1‐day post‐bioprinting, data show the mean ± SD of n = 3 independent experiments. (c) Immunocytochemistry of iPSC‐derived astrocytes bioprinted in the 3D matrix and shows a maximum intensity projection of an 80 μm z‐stack. Scale bar = 20 μm. DAPI, 4′,6‐diamino‐2‐phenylindole dihydrochloride; GFAP, glial fibrillary acidic protein; iPSC, induced pluripotent stem cell.