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. 2023 Jul 3;120(10):3079–3091. doi: 10.1002/bit.28470

Figure 5.

Figure 5

Viability of human iPSC‐derived brain microvascular endothelial‐like cells (iBMECLs) bioprinted in 2D or 3D matrices under different media conditions. Comparison of raw fluorescence units (RFU) after 4 h incubation with Cell Titer Blue in 2D cultures at day 1 (a) and day 7 (b), and in 3D cultures at day 1 (c), day 7 (d), and day 28 (e). Graphs are presented as mean ± SD of n ≥ 3 independent experiments. (a)–(e) are compared using a one‐way ANOVA with Tukey's multiple comparisons tests. *p < 0.05, **p < 0.01, and ***p < 0.001. AM, Astrocyte Medium; ANOVA, analysis of variance; EM, endothelial medium; iPSC, induced pluripotent stem cell; ROCKi, rho kinase inhibitor.