Fig. 3. Top validated amides (the amine used in the peptide bond forming reaction shown for clarity) of crude assay inhibition. IC50 values of the resulting amide shown below each compound. Selection criteria for compounds is listed. (Left panel) Top 5 putative and uncovered false negatives. (Right panel) Top 5 D2B hits from crude fluorogenic assay results (true positives). Deleterious aniline motif is highlighted in pink.
