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. 1998 Mar;72(3):2364–2372. doi: 10.1128/jvi.72.3.2364-2372.1998

FIG. 3.

FIG. 3

Characterization of DI9c as an autonomous RNA replicon. (A) RNase protection analysis of MDBK and BHK-21 cells transfected with DI9c 24 h p.t. The protected fragments were analyzed on a 10% polyacrylamide–7 M urea gel. Lanes: p, input sense (−) and antisense (+) probes (1/10 of the experimental input was loaded); 1, RNase protection with cytoplasmic RNA obtained from MDBK cells transfected with DI9c using sense (−) and antisense (+) probes; 2, RNase protection with cytoplasmic RNA obtained from BHK-21 cells transfected with DI9c; 3, MDBK cells with DI9cΔ3′; 4, MDBK cells with DI9cpm1. (B) IF analysis of MDBK and BHK-21 cells transfected with DI9c, DI9cΔ3′, or DI9cpm1 24 h p.t. 1, MDBK cells with DI9c (magnification, ×100); 2 and 3, MDBK cells with DI9c (magnification, ×400); 4, BHK-21 cells with DI9c (magnification, ×100); 5, BHK-21 cells with DI9c (magnification, ×400); 6, MDBK cells with DI9cΔ3′ (magnification, ×100); 7, MDBK cells with DI9cpm1 (magnification, ×400); 8, BHK-21 cells with DI9cΔ3′ (magnification, ×100); 9, BHK-21 cells with DI9cpm1 (magnification, ×400).