FIGURE 3.

Cultivation of A. pullulans NRRL 62042 with different nitrogen sources: Comparison of cell dry weight over the first 48 h (A), and 240 h (B), polyol lipid (C), glucose (D), pullulan (E), and pH (F) over time by using 126 g L⁻¹ glucose and different nitrogen sources (0.9 g L⁻¹ yeast extract (YE) and 1.5 g L⁻¹ peptone (PEP), 2.01 g L⁻¹ NaNO3, 1.56 g L⁻¹ (NH₄)₂SO₄, 1.27 g L⁻¹ NH₄Cl, or 0.95 g L⁻¹ NH₄NO₃). Cultivations were performed with A. pullulans NRRL 62042 in 24 deep-well microtiter plates with 2 mL filling volume, 30°C, and 300 rpm. Data show the mean of triplicates with ±standard deviation.