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. 2024 Mar 6;12:1379707. doi: 10.3389/fbioe.2024.1379707

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Copyright © 2024 Haala, Dielentheis-Frenken, Brandt, Karmainski, Blank and Tiso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cultivation of A. pullulans NRRL62042 with different phosphate and nitrogen concentrations: Polyol lipid production using the optimized MLP medium with different K₂HPO₄ concentrations after 240 h (A). Polyol lipid production and glucose consumption over time using the optimized MLP medium with different NH₄NO₃ concentrations (B). Cultivations were performed with A. pullulans NRRL 62042 in 24 deep-well microtiter plates with 2 mL filling volume, 30 °C, and 300 rpm. Data show the mean of triplicates with ±standard deviation. Statistically significant differences in polyol lipid production were determined by Welch’s t-test and are indicated as * (p-value ≤0.5) and *** (p-value ≤0.001).