TABLE 3.
List of factors that are used in central composite design: After using a two-level factorial design to identify glucose and K2HPO4 as factors significantly influencing the polyol lipid titer, these were selected as factors in the central composite design. Based on the given low and high concentrations, the center and star points were determined and the experimental plan was designed.
| Factor | Unit | Low conc | Center point | High conc | Star point max | Star point min |
|---|---|---|---|---|---|---|
| Glucose | g L-1 | 120 | 210 | 300 | 337.3 | 82.7 |
| K2HPO4 | g L-1 | 1.4 | 4.7 | 8.0 | 9.37 | 0.03 |