TABLE 7.
Comparison of performance parameters using A. pullulans NRRL 62042: Comparison of performance parameters (maximal growth rate (µmax), polyol lipid, cell dry weight (CDW) after growth (48 h) and after 240 h, pullulan, product-to-substrate yield (YP/S), product-to-biomass yield (YP/X) after growth (48 h) and after 240 h, and space-time yield (STY)) between minimal liamocin production (MLP with 126 g L−1 glucose and 1 g L−1 K2HPO4) and optimized minimal liamocin production medium (oMLP with 208 g L−1 and 6.3 g L−1 K2HPO4) cultivating A. pullulans NRRL 62042 on MTP (24-well microtiter plates with 2 mL filling volume, 30°C, 300 rpm at 50 mm for 10 days, n = 3) and bioreactor (s vessel with 0.7 L filling volume, 30°C, 30% DO controlled with 300–1,200 rpm stirring rate, 0.5 vvm, n = 2) scale.
| MLP | Optimized MLP | ||||
|---|---|---|---|---|---|
| MTP | Bioreactor | MTP | Bioreactor | ||
| µmax | [h−1] | 0.12 | 0.11 | - | 0.18 |
| cpolyol lipid | [gPL L−1] | 30.8 | 28.7 | 48.1 | 41.3 |
| cCDW, 48 h | [gCDW L−1] | 9.1 | 8.2 | - | 17.2 |
| cCDW, 240 h | [gCDW L−1] | 12.9 | 9.4 | - | 27.0 |
| cpullulan | [gpullulan, max L−1] | 9.3 | 27.1 | - | 50.5 |
| YP/S | [gPL gGlc − 1] | 0.24 | 0.23 | 0.23 | 0.20 |
| YP/X 48 h | [gPL gCDW −1] | 3.6 | 3.6 | - | 2.4 |
| YP/X 240 h | [gPL gCDW −1] | 2.4 | 3.1 | - | 1.5 |
| STY | [gPL L−1 h−1] | 0.13 | 0.12 | 0.20 | 0.19 |