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Immunoblot pattern of serum specific for Ad capsid components. Rec-Ad (107 PFU/well) was deposited, and the viral proteins were separated by SDS-PAGE. Immunoblots were incubated with the different sera, collected on day 0 (D0) to day 90 (D90), diluted at 1/100, and washed with HRP-labeled anti-human Ig conjugate. The capsid proteins Hx, Pb, and Fi are indicated. The controls contained rabbit anti-Fi, rabbit anti-Pb, and mouse anti-Hx antibodies.
