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. 2024 Feb 29;15:1315283. doi: 10.3389/fimmu.2024.1315283

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Copyright © 2024 Cianciotti, Magnani, Ugolini, Camisa, Merelli, Vavassori, Potenza, Imparato, Manfredi, Abbati, Perani, Spinelli, Shifrut, Ciceri, Vago, Di Micco, Naldini, Genovese, Ruggiero and Bonini

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TCR_ED-IR_KO cells display a unique transcriptional profile upon target recognition. Volcano plot showing significant down- (green) and up- (red) regulated genes (A) and curated Gene ontology terms (biological process-(B) in stimulated TCR_ED-LAG-3_KO vs stimulated TCR_ED-IR_COMP cells. (C) UpSet analysis showing shared (connected dots in the lower panel) or unique (not-connected dots in the lower panel) differentially expressed genes in stimulated vs unstimulated TCR_ED-IR_COMP, TCR_ED-LAG-3_KO, TCR_ED-TIM-3_KO, TCR_ED-2B4_KO cells. (D) Heatmap showing the level of expression of selected genes among the 757 uniquely differentially expressed genes in TCR_ED-LAG-3_KO upon target recognition (red box in panel (C). (E) Treeplot of Gene Ontology (Biological Process) terms enriched uniquely in TCR_ED-LAG-3_KO upon target recognition (red box in panel (C)). Data are shown for 3 biological replicates for each cellular product, generated from 3 matched healthy donors. MM1.s A2^posESO-1^pos multiple myeloma cells were used as antigen source for T cell stimulation.