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. 2024 Mar 6;15:1335246. doi: 10.3389/fphar.2024.1335246

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Copyright © 2024 Atanasova, Perkins, Ratnayake, Jiang, Chen, Schmittgen and Luesch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of viable ducts and acinar clusters in trichostatin A (TSA)-treated organoids. Percent duct/cluster distribution (± SD) of live objects at 72 h post treatment with positive control TSA as a function of the concentration in ADM inhibition (wildtype mouse organoids, Z’ = 0.61) (A) and ADM reversal (Cre-mice organoids, Z’ = 0.56) (B) assays. Representative images at 1 µM TSA for ADM inhibition (C) and ADM reversal (D) assays. Scale bars represent 100 µm. Single biological replicate of quadruplicate technical replicates, mean ± SD.