FIGURE 10.

Testing of potency of largazole homodimer in ADM reversal mode (Cre mice organoids) at 72 h post treatment. (A) Structures of Largazole homodimer and justification for drug selection. Prodrug strategy was applied, which increases stability of the compound and also generates two equivalents of the active species, leading to increased potency compared to the parent compound. (B) Dose response study of largazole homodimer in ADM reversal assay mode. (Left) Percent live clusters from all live objects. IC₅₀ value was calculated using GraphPad Prism 9 software based on quadruplicate experiments. (Right) Representative images of morphological changes observed with the homodimer at select doses in ADM reversal assay mode compared to vehicle control (0.5% DMSO). Scale bars represent 100 µm. Single biological replicate of quadruplicate technical replicates, mean ± SD.