FIGURE 12.

Validation of acinar and ductal gene expression following ADM reversal in KC organoids. KC mouse ADM organoids were treated with TSA, largazole, largazole homodimer, FK228, or chaetocin at varying concentrations for 2 or 3 days immediately following 2 days of ADM transdifferentiation. Cultures were collected following the 48 or 72 h treatments and gene expression was analyzed by qRT-PCR. Data are normalized to the 48 h DMSO control and are presented relative to 18S rRNA. Mean ± SD from two independent experiments.