Fig 5. ONT sequencing for Mpeg1-cre knock-in project.
The figure summarises the outcome of ONT sequencing of (A) a founder animal (G0, Mpeg1-cre-80) and (B) its offspring (G1, Mpeg1-cre-80.1c) visualised with IGV. The alignment reflects the noisy nature of the method with errors distributed across the length of the sequenced segment. Note the complete alignment of reads to designed mutant sequences for both founder and offspring (grey histograms). The dip in sequence coverage coincides with the presence of homopolymer repeats (Mpeg1-cre-80, coloured framed, S7 Fig).