Extended Data Fig. 2 |. The JunB Chaser mouse model was generated to show the presence of JunB-expressing adipocytes in fat, related to Fig. 2.

a. The generation strategy for JunB^CreERT2 mice as described in the genomic structure. An internal ribosome entry site (IRES) fused to a CreERT2 fusion gene was inserted downstream of the internal stop codon of Junb gene. b. Representative tissue distribution of iCre and Junb mRNA in adult JunB^CreERT2 transgenic mice as determined by qPCR. c. Immunofluorescence staining of YFP⁺ cells in liver and pancreas of JunB^CreERT2 mice. Differentiated YFP⁺ brown adipocytes were present during primary adipogenesis by imaging (d) and flow cytometry (e) in Primary brown adipocytes of JunB^CreERT2 mice. f. JunB⁺ adipocytes were increased by the treatment of 20 ng/mL TNFα or 20 ng/mL IL-6 for 24 hrs post primary adipogenesis. g. Quantification of JunB-expressing adipocytes in Figure f. h. JunB⁺ adipocytes were increased by the treatment of 20 ng/mL TNFα or 20 ng/mL IL-6 for 24 hrs post primary adipogenesis. Preadiocytes were isolated from AdipoChaser-YFP mice and differentiated into adipocytes. i. Quantification of JunB-expressing adipocytes in Figure h. All data in this Figure were analyzed by unpaired two-sided T-Test.