FIG. 2.

Cross-recognition of HIV-2 and HIV-1 Gag protein by HIV-2 Gag-specific CTL lines. Effector cells were tested against autologous target cells infected with rVV expressing the Gag proteins of HIV-2 and HIV-1 clades A1, A2, B, and C (see the legend to Fig. 1). (A) Patients with broad cross-reactivity; (B and C) patients with limited (B) and no (C) cross-reactivity. HLA class I profiles of the different subjects are shown at the top of each panel. The E/T ratio varied according to the number of cells that grew from each culture but was usually between 20:1 and 50:1. Recognition of different HIV-1 clades was regarded as positive if lysis of different HIV-1 Gag rVV was more than 10% above the level of control vaccinia virus.