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. 2024 Feb 21;627(8004):628–635. doi: 10.1038/s41586-024-07098-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — A) Flow cytometry based immune cell profiling of the colonic lamina propria from Control (LysM Cre +/−) and SCD2 cKO (LysM Cre +/− Scd2^flox/flox) naïve male mice (n = 8−9 per genotype). B) ELISA analysis of IL-12B (IL12p40) from colonic explants of naïve Control (LysM Cre +/−) and SCD2 cKO (LysM Cre +/− Scd2^flox/flox) male mice incubated ex vivo for 24 h. (n = 6−9 per genotype). C) Flow cytometry based immune cell profiling of the colonic lamina propria from Control (LysM Cre +/−) and SCD2 cKO (LysM Cre +/− Scd2^flox/flox) female mice harvested on day 12 of DSS challenge (n = 9 per genotype). D) Weight loss measured during DSS-induced colitis in Control (LysM Cre +/−), SCD2 conditional heterozygous (Het) (LysM Cre +/−, Scd2 flox/WT) and SCD2 cKO (LysM Cre +/− Scd2 flox/flox) female mice (n = 6-11 per group). Red stars indicated statistical differences between Control and SCD2 cKO mice, while black stars represent statistical differences between Control and Het mice. There is no statistical difference in weight loss between SCD2 cHet and SCD2 cKO animals. Error bars represent Standard Error of the Mean (SEM). E) ELISA analysis of lipocalin from feces of Day 0: heterozygous (het) vs IL-10R KO mice, (all male mice). F) ELISA analysis of lipocalin from feces of Day 0: IL-10 KO female mice versus Day 14: IL-10 KO mice gavaged with BSA or BSA-18:1 (all female mice). G) Flow cytometry based myeloid cell profiling of the colonic lamina propria from heterozygous (het) mice gavaged with BSA, IL-10R KO mice gavaged with BSA or IL-10R KO mice gavaged with BSA-24:1 (all male mice). H) Flow cytometry based myeloid cell profiling of the colonic lamina propria from WT mice gavaged with BSA, IL-10 KO mice gavaged with BSA or IL-10 KO mice gavaged BSA-18:1 (all female mice). I) Flow cytometry based myeloid cell profiling of the colonic lamina propria from heterozygous (het) mice gavaged with BSA, IL-10R KO mice gavaged with BSA or IL-10R KO mice gavaged with BSA-16:0 (all male mice). All experiments are reported as means ± SD from 3 independent experiments, unless noted otherwise. *P < 0.05; **P < 0.01, ***P < 0.005 (two-tailed unpaired Student’s t test).

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