Fig. 1. SNARE proteins do not restrict targeting destination.

Colocalization between injected proteoliposomes (PLs) reconstituted with four early endosomal (EE) SNAREs (including syntaxin 13, vti1a, syntaxin 6, and VAMP4) (4-EE-SNARE PL) and/or late endosomal SNAREs (syntaxin 7, vti1b, syntaxin 8, VAMP) (4-LE-SNARE PL) and endogenous markers. In these experiments, all values were normalized to the degree of colocalization observed in control injections using protein-free liposomes containing a corresponding lipid composition with no further additions (see methods). The data show mean values ± SEM of 3–9 independent experiments, with each individual measurement being represented by a black dot. At least 100 injected vesicles were analyzed for the colocalization with each organelle marker in each experiment. Stars indicating significance: *P < 0.05, **P < 0.01, ***P < 0.001, all determined by 1-way ANOVA with the Tukey multiple comparison test. Source data are provided as a Source Data file.