Skip to main content
. 2024 Mar 20;15:2484. doi: 10.1038/s41467-024-46785-9

Fig. 1. TP63 suppresses IFNγ response signaling in SCC tumors.

Fig. 1

A Scheme of RNA-seq analysis in SCC primary tumors and cell lines. Right upper: Hallmark pathway enrichment analysis showing the top 10 pathways that are negatively correlated with the expression of TP63. Right lower: gene set enrichment analysis (GSEA) plots revealing significant enrichment of IFNγ and IFNα response pathways in TP63-low expressed SCC cells. Data were from TCGA (n = 1077) and CCLE (n = 112), respectively. ESCC: esophageal squamous cell carcinoma; HNSC: head and neck squamous cell carcinoma; LUSC: lung squamous cell carcinoma. B GSEA revealing significant enrichment of upregulated genes in IFNγ and IFNα response pathways upon knockdown of TP63 expression in 3 SCC cell lines. RNA-seq data were from in-house (GSE106564) and public datasets (GSE88833 and GSE4975). C Venn diagram representing TP63 negatively regulated IFNα/γ response genes in three types of SCCs. 23 overlapped ISGs are listed below. D, E qRT-PCR analysis showing relative mRNA levels of TP63 (D) and the 23 ISGs (E) in human (TT) and murine SCC (MOC22) cells expressing non-targeting control (Scramble) or TP63-targeting shRNA (shTP63) pulsed with IFNγ (100 ng/mL) for 48 h. Data represent mean ± SD, n = 3 biologically independent experiments. F Western blotting analysis showing the protein levels of TP63 and representative ISGs of E in TT and MOC22 cells expressing non-targeting control (Scramble) or TP63-targeting shRNA (shTP63) pulsed with IFNγ (100 ng/mL) for 48 h. The results were repeated with three biologically independent experiments in two cell lines. Source data and exact P values for Fig. 1D–F are provided as a Source Data file.