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. 2024 Mar 20;15:2484. doi: 10.1038/s41467-024-46785-9

Fig. 7. Antagonistic transcription regulation between TP63 and STAT1 in SCCs.

Fig. 7

A Density plots of ChIP-seq signals of TP63 and STAT1 at ± 3 kb windows flanking the center of TP63 peaks. Color bars at the bottom show reads-per-million-normalized signals. Data were from GSE46837, GSE106563, and GSE148920. B Pie chart showing genome-wide distribution of the regions uniquely- or co-occupied by TP63 and STAT1. C Representative top TF motif sequences enriched at TP63 or/and STAT1 uniquely- or co-occupied loci revealed by de novo motif analysis. D, E Gene Ontology (GO) functional categories of TP63 (D) or STAT1 (E) uniquely occupied peak-assigning genes in A. F, G IGV tracks from ChIP-seq data of indicated factors at either TP63 (F) or STAT1 (G) gene loci. Gray shadows highlighting enhancer or promoter elements that are co-occupied by TP63 and STAT1. Black font sequences: TP63 binding motif; Red font sequences: STAT1 binding motif. H, I Relative luciferase activity of pGL3-enhancer (1st Empty), pGL3-enhancer+STAT1 promoter, pGL3-promoter (2nd Empty), pGL3-promoter+ STAT1 enhancer, pGL3-promoter+e8 upon stimulation with IFNγ (100 ng/mL) +/− Fludarabine (10 µM) in TP63-high TE5 cells (H) or over-expression of TP63 in TP63-low TE1 cells (I). Relative luciferase activity comparison of Control vs. IFNγ, IFNγ vs. IFNγ + Fludarabine in H: P = 0.0251 (*) and P = 0.0492 (*) for STAT1-promoter group, P = 0.0037 (**) and P = 0.0213 (*) for STAT1-enhancer group, P = 0.0033 (**) and P = 0.0318 (*) for e8 group. Relative luciferase activity comparison of Control vs. OE-TP63 in I: P = 0.0137 (*) for STAT1-promoter group, P = 0.0068 (**) for STAT1-enhancer group, P = 0.0016 for e8 group. C–E Statistical analysis was determined by hypergeometric test. H, I Data represent mean ± SD of three biologically independent experiments. P values were determined using a two-sided t -test. Source data are provided as a Source Data file.