Figure 3.

More MAC⁺ cells in all glaucoma groups. (A) Retinae were stained with an antibody against MAC (terminal complement complex; red) and cell nuclei were labelled with DAPI (blue). An antibody against Iba1 (microglia/macrophages; green) was used to show co-staining of MAC and Iba1. Mostly, MAC⁺ cells were not co-stained with microglia/macrophages (detailed images). (B) Regarding cell counts in the GCL, significantly more MAC⁺ deposits were noted in WT+ONA (p=0.002), CTGF (p<0.001), and CTGF+ONA mice (p=0.003) compared to WT ones. (C) In the IPL, the number of MAC⁺ cells was similar within all groups. (D) In the INL, significantly more MAC⁺ cells were observed in WT+ONA (p=0.003), CTGF (p=0.008), and CTGF+ONA mice (p=0.037) compared to WT animals. (E) The mRNA expression levels of Hc were not altered within all groups. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Values in (B–D) are mean ± SEM and in (E) median ± quartile ± minimum/maximum. The dotted line in (E) represents the relative expression of the WT group. Scale bars: 20 µm, scale bar in detailed images: 10 µm. For immunohistology: n=7 retinae/group, for RT-qPCR: n=4 samples/group. *p<0.050; **p<0.010, and ***p<0.001 vs. WT.