Figure 7.

More T-cells in the GCL of CTGF+ONA mice. (A) Retinae of all groups were labelled with an antibody against CD3 to detect pan T-cells (green). DAPI counterstained cell nuclei (blue). (B) Additionally, an antibody against Iba1 (microglia/macrophages; red) was used to investigate possible co-staining of CD3 (green) and Iba1. Cell nuclei were labelled with DAPI (blue). The staining revealed that T-cells were predominantly not co-labelled with Iba⁺ microglia/macrophages. (C) In the GCL, the number of T-cells remained unchanged in WT+ONA, CTGF, and CTG+ONA mice compared to WT ones. Significantly more CD3⁺ cells were noted in CTGF+ONA mice compared to the CTGF group (p=0.025). (D) The number of CD3⁺ T-cells was not altered within the groups counted in the IPL. (E) Also, no changes could be detected in the INL regarding the number of T-cells. (F) Flow cytometry of CD4⁺ T-helper cells revealed no alterations within all groups. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Values in (C–E) are mean ± SEM and in (F) mean ± interquartile range ± range. Scale bars: 20 µm, scale bar in detailed images: 10 µm. For immunohistology: n=7 retinae/group, for flow cytometry: n=4 samples/group. ^¥p<0.050 vs. CTGF.