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. 2024 Mar 7;15:1296178. doi: 10.3389/fimmu.2024.1296178

Table 1.

Primary antibodies listed in alphabetical order and corresponding secondary antibodies used for immunohistology.

Primary antibodies Corresponding secondary antibodies
Antibody Company Order number Concentration stock solution Dilution Antibody Company Dilution Order number Concentration stock solution
C1q Abcam, Cambridge, UK ab182451 Not stated 1:500 Donkey anti-rabbit Alexa Fluor 555 Invitrogen, Carlsbad, CA, USA 1:500 A31572 2.0 mg/ml
C3 Cedarlane, Burlington, ON, Canada CL7334Ap 100 µg/ml 1:700 Goat anti-rabbit Cy3 Linaris, Dossenheim, Germany 1:500 ZRV1159 2.0 mg/ml
CD3-FITC eBioscience, Waltham, MA, USA 14-0031-82 500 µg/ml 1:100 None (directly labelled)
Iba1 Synaptic Systems, Göttingen, Germany 234009 1000 µg/ml 1:500 Donkey anti-chicken Alexa Fluor 488 Jackson Immuno Research, Ely, UK 1:500 703-545-155 1.5 mg/ml
Donkey anti-chicken Cy3 Millipore, Burlington, MA, USA 1:500 AP194C 500 µg
MAC Thermo Fisher, Waltham, MA, USA BS-2673R 1000 µg/ml 1:200 Donkey anti-rabbit Alexa Fluor A555 Invitrogen, Carlsbad, CA, USA 1:500 A31572 2.0 mg/ml