Table 2.

List of oligonucleotides used for mRNA expression analysis in retinae, while Actb and Ppid served as reference genes.

Gene	Forward (F) and reverse (R) oligonucleotides	GenBank acc. no.	Amplicon size
Actb-F Actb-R	ctaaggccaaccgtgaaag accagaggcatacagggaca	NM_007393.5	104 bp
C1qa-F C1qa-R	cgggtctcaaaggagagaga tcctttaaaacctcggatacca	NM_007572.2	71 bp
C1qb-F C1qb-R	aggcactccagggataaagg ggtcccctttctctccaaac	NM_009777.3	80 bp
C1qc-F C1qc-R	atggtcgttggacccagtt gagtggtagggccagaagaa	NM_007574.2	75 bp
C3-F C3-R	accttacctcggcaagtttct ttgtagagctgctggtcagg	NM_009778.3	75 bp
Cd68-F Cd68-R	tgatcttgctaggaccgctta taacggcctttttgtgagga	NM_001291058.1	66 bp
Cfb-F Cfb-R	ctcgaacctgcagatccac tcaaagtcctgcggtcgt	M57890.1	112 bp
Hc-F Hc-R	tgacaccaggcttcagaaagt agttgcgcacagtcagctt	XM_017315669.2	69 bp
Itgam-F Itgam-R	cctgtccctggctgtttcta accggagccatcaatcaaga	NM_001082960.1	208 bp
Masp2-F Masp2-R	ggcggctactattgctcct aacacctggcctgaacaaag	NM_001003893.2	86 bp
Nos2-F Nos2-R	ctttgccacggacgagac tcattgtactctgagggctgac	NM_010927.4	66 bp
Ppid-F Ppid-R	aaggatggcaaggattgaaa ctttaagcaattctgcctgga	NM_026352	95 bp
Tgfb-F Tgfb-R	aggaggtttataaaatcgacatgc tgtaacaactgggcagacagttt	XM_006497136.3	65 bp
Tnf-F Tnf-R	ctgtagcccacgtcgtagc ttgagatccatgccgttg	NM_013693.3	97 bp

The predicted amplicon sizes are given. F, forward; R, reverse; acc. no., accession number; bp, base pair.